Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO
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چکیده
منابع مشابه
Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer
Early in F plasmid conjugative transfer, the F relaxase, TraI, cleaves one plasmid strand at a site within the origin of transfer called nic. The reaction covalently links TraI Tyr16 to the 5'-ssDNA phosphate. Ultimately, TraI reverses the cleavage reaction to circularize the plasmid strand. The joining reaction requires a ssDNA 3'-hydroxyl; a second cleavage reaction at nic, regenerated by ext...
متن کاملAnalysis of the traLEKBP sequence and the TraP protein from three F-like plasmids: F, R100-1 and ColB2.
The sequence of a region of the F plasmid containing the traLEKBP genes involved in plasmid transfer was compared to the equivalent regions of two IncFII plasmids, R100-1 and ColB2. The traLEK gene products of all three plasmids were virtually identical, with the most changes occurring in TraE. The TraB genes were also nearly identical except for an 11-codon extension at the 3' end of the R100-...
متن کاملCharacterization of the F-plasmid conjugative transfer gene traU.
We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped between traW and trbC. It encodes a 330-amino-acid, Mr36,786 polypeptide that is pro...
متن کاملSwapping single-stranded DNA sequence specificities of relaxases from conjugative plasmids F and R100.
Conjugative plasmid transfer is an important mechanism for diversifying prokaryotic genomes and disseminating antibiotic resistance. Relaxases are conjugative plasmid-encoded proteins essential for plasmid transfer. Relaxases bind and cleave one plasmid strand site- and sequence-specifically before transfer of the cleaved strand. TraI36, a domain of F plasmid TraI that contains relaxase activit...
متن کاملf-DERIVATIONS AND (f; g)-DERIVATIONS OF MV -ALGEBRAS
Recently, the algebraic theory of MV -algebras is intensively studied. In this paper, we extend the concept of derivation of $MV$-algebras and we give someillustrative examples. Moreover, as a generalization of derivations of $MV$ -algebraswe introduce the notion of $f$-derivations and $(f; g)$-derivations of $MV$-algebras.Also, we investigate some properties of them.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1987
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.169.2.619-623.1987